Rabbit monoclonal antibody raised against 25-OH Vitamin D3/RM3,D3/RMH04.
Immunogen:
25-OH Vitamin D3 Antibody-antigen Complex
Host:
Rabbit
Specificity:
This antibody reacts to 25-OH Vitamin D3/RM3 or 25-OH Vitamin D3/ RMH04 Complexes. It does not cross-react with Vitamin D3, Vitamin D2, and 25-OH Vitamin D2. It also does not recognize free 25-OH Vitamin D3, RM3 or RMH04.
Form:
Liquid
Purification:
Protein A purification
Isotype:
IgG
Recommend Usage:
ELISA (0.5 ug/mL-2 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide).
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of 25(OH) Vitamin D Standard Curve: ELISA plate was coated with 100 ng capture antibody RMH04 at concentration of 1ug/mL. A serial dilution from 150 ng/mL was performed using 25-OH Vitamin D3/RM3 or 25-OH Vitamin D3/ RMH04 monoclonal antibody, clone RM428 was used as a detection antibody.