NEFL monoclonal antibody, clone DA2
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More Files
- More Functions
Western Blot (Tissue lysate)
Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The lane shows blot of this material stainted with NEFL monoclonal antibody, clone DA2 (Cat # MAB2321).
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Specification
Product Description
Mouse monoclonal antibody raised against native NEFL.
Immunogen
Native purified pig NEFL.
Host
Mouse
Theoretical MW (kDa)
68
Reactivity
Birds, Human, Mammals
Specificity
Specifically recognizes the light neurofilament subunit or NF-L (~68 KDa).
Form
Liquid
Isotype
IgG1, kappa
Recommend Usage
Immunohistochemistry (1:250)
Immunofluorescence (1:100-1:500)
Western Blot (1:5000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.09% sodium azide
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The lane shows blot of this material stainted with NEFL monoclonal antibody, clone DA2 (Cat # MAB2321).Immunohistochemistry
Immunofluorescence
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Gene Info — LOC733642
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Publication Reference
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Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells.
Shaw G, Morse S, Ararat M, Graham FL.
FASEB Journal 2002 Apr; 16(8):869.
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Identification of a tektin-like protein associated with neurofilaments in the developing chick nervous system.
Edson KJ, Linck RW, Letourneau PC.
Journal of Neuroscience Research 1991 Sep; 30(1):105.
Application：IEM, IF, WB-Ti, Chicken, Chicken brain, Chicken spinal cord.
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Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells.
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