NEFH monoclonal antibody, clone NAP4
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More Files
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Western Blot (Tissue lysate)
Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The lane shows blot of this material stainted with NEFH monoclonal antibody, clone NAP4 (Cat # MAB2320).
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Specification
Product Description
Mouse monoclonal antibody raised against native NEFH.
Immunogen
Native purified neurofilaments from pig spinal cord.
Host
Mouse
Theoretical MW (kDa)
180-220
Reactivity
Birds, Human, Mammals
Specificity
Specifically recognizes the phosphorylated variant of NF-H subunit (~180-220 KDa), showing some weaker reactivity with phosphorylated forms of NF-M.
Form
Liquid
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against Native Purified Protein.
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:1000)
Western Blot (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM sodium phosphate buffer, 250 mM NaCl, pH 7.6 (15 mg/mL BSA, 5 mM sodium azide, 0.01% thimerosal)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The lane shows blot of this material stainted with NEFH monoclonal antibody, clone NAP4 (Cat # MAB2320).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Caveolin-1 deficiency increases cerebral ischemic injury.
Jasmin JF, Malhotra S, Singh Dhallu M, Mercier I, Rosenbaum DM, Lisanti MP.
Circulation Research 2007 Mar; 100(5):721.
Application：IF, Rat, Rat ischemic hemispheres.
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A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H.
Harris J, Ayyub C, Shaw G.
Journal of Neuroscience Research 1991 Sep; 30(1):47.
Application：Func, Fusion protein.
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Caveolin-1 deficiency increases cerebral ischemic injury.
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