DDDDK tag monoclonal antibody, clone RM399
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot of 293T cells. Lane 1: transfected or Lane 2: non- transfected with a DNA construct encoding DDDDK tag fusion protein, using DDDDK tag monoclonal antibody clone RM399 at 0.001 ug/mL.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mixture of DDDDK-Tag peptides.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a mixture of a peptide with DDDDK-Tag at the N-terminal and another peptide with DDDDK-Tag at the C-terminal.
Specificity
This antibody reacts to recombinant proteins containing DDDDK tag. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Western Blot (0.001 ug/mL - 0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot of 293T cells. Lane 1: transfected or Lane 2: non- transfected with a DNA construct encoding DDDDK tag fusion protein, using DDDDK tag monoclonal antibody clone RM399 at 0.001 ug/mL.
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