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Last updated: 2016/12/11

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GAPDH monoclonal antibody, clone 1D4

  • Catalog # : MAB2319
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against GAPDH.
  • Immunogen:
  • Pig GAPDH.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 37
  • Reactivity:
  • Birds, Bovine, Human, Mouse, Pig, Rat
  • Specificity:
  • On Western Blot reveals a prominent ~37 KDa band.
  • Form:
  • Liquid
  • Isotype:
  • IgM
  • Quality Control Testing:
  • Antibody Reactive Against GAPDH.
  • Recommend Usage:
  • Immunofluorescence (1:100)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In tissue culture supernatant (10 mM sodium azide)
  • Storage Instruction:
  • Store at 4°C for short term. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Sciatic nerves of mouse wild type (1), heterozygous (2) and homozygous (3) for knock out of peripheral myelin protein 21 (pmp21) were homogenized in SDS-PAGE sample buffer and run out for western blots.
    Blots were probed with GAPDH monoclonal antibody, clone 1D4 (Cat # MAB2319) to glyceraldehyde 3-phosphate dehydrogenase (GAPDH).
    Antibody was used at dilution of 1 : 1,000.
    Signal was revealed in a few seconds with chemiluminescence, indicating that lower antibody concentrations would also have worked well.
    Note the sharp clear band at 38 KDa, the expected molecular weight for GAPDH.
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Human neuroblastma line Sh-SY5Y stained with GAPDH monoclonal antibody, clone 1D4 (Cat # MAB2319) (green) and an anti-NF-H (red), counterstained with a fluorescent DNA probe (blue).
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunocytochemistry
  • Gene Information
  • Gene Name:
  • GAPDH
  • Gene Alias:
  • GAPD
  • Gene Description:
  • glyceraldehyde-3-phosphate dehydrogenase
  • Other Designations:
  • -
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