Sciatic nerves of mouse wild type (1), heterozygous (2) and homozygous (3) for knock out of peripheral myelin protein 21 (pmp21) were homogenized in SDS-PAGE sample buffer and run out for western blots. Blots were probed with GAPDH monoclonal antibody, clone 1D4 (Cat # MAB2319) to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Antibody was used at dilution of 1 : 1,000. Signal was revealed in a few seconds with chemiluminescence, indicating that lower antibody concentrations would also have worked well. Note the sharp clear band at 38 KDa, the expected molecular weight for GAPDH.
Human neuroblastma line Sh-SY5Y stained with GAPDH monoclonal antibody, clone 1D4 (Cat # MAB2319) (green) and an anti-NF-H (red), counterstained with a fluorescent DNA probe (blue).