Histone H3 (trimethyl K9) monoclonal antibody, clone RM389
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: recombinant Histone H3.3, Lane 2: acid extracts of HeLa cells with Histone H3 (trimethyl K9) monoclonal antibody, clone RM389 (Cat # MAB23170) under 0.5 ug/mL working concentration. showed a band of Histone H3 trimethylated at Lysine 9 (K9me3) in HeLa cells.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Designer Nucleosomes (dNucs) (Recombinant Human Nucleosome with H3 K4, 9, 27, 36 and H4 K20 Mono-, Di-, and Tri-Methylation), Histone H3 (trimethyl K9) monoclonal antibody, clone RM389 (Cat # MAB23170) (0.5 ug/mL).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human histone H3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic trimethyl peptide corresponding to residues surrounding Lys9 of human histone H3.
Reactivity
Human
Specificity
This antibody reacts to Histone H3 trimethylated at Lysine 9. No cross reactivity with monomethylated Lysine 9, dimethylated Lysine 9, or other methylations in histone H3.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ELISA (1 ug/mL-10 ug/mL)
Multiplex (0.5 ug/mL-2 ug/mL)
Western Blot (0.5 ug/mL-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: recombinant Histone H3.3, Lane 2: acid extracts of HeLa cells with Histone H3 (trimethyl K9) monoclonal antibody, clone RM389 (Cat # MAB23170) under 0.5 ug/mL working concentration. showed a band of Histone H3 trimethylated at Lysine 9 (K9me3) in HeLa cells.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Designer Nucleosomes (dNucs) (Recombinant Human Nucleosome with H3 K4, 9, 27, 36 and H4 K20 Mono-, Di-, and Tri-Methylation), Histone H3 (trimethyl K9) monoclonal antibody, clone RM389 (Cat # MAB23170) (0.5 ug/mL).
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