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Last updated: 2021/8/8

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GFP monoclonal antibody, clone 9F9.F9 (Texas Red) 

  • Catalog # : MAB23145
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant GFP. The antibody is conjugated with Texas Red.
  • Immunogen:
  • Recombinant fusion protein corresponding to full length amino acid sequence (246 amino acids) derived from the jellyfish Aequorea victoria GFP.
  • Host:
  • Mouse
  • Specificity:
  • This antibody reactivity is observed against recombinant Green Fluorescent Protein. No reaction is seen against RFP.
  • Form:
  • Lyophilized
  • Conjugation:
  • Texas Red
  • Purification:
  • This antibody was prepared from tissue culture supernatant by protein A affinity chromatography.
  • Isotype:
  • IgG1, kappa
  • Conjugation Note:
  • FP Value: 3.0 moles Texas Red per mole of IgG
  • Recommend Usage:
  • ELISA (>1:20000)
    Immunofluorescence (1:500-1:2500)
    Western Blot (>1:10000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 20 mM potassium phosphate, 150 mM NaCl, pH 7.2 (10 mg/mL BSA, 0.01% (w/v) sodium azide).
  • Storage Instruction:
  • Store vial at 4°C prior to restoration.
    After reconstitution with 1.0 mL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
    Aliquot to avoid repeated freezing and thawing.
    Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Immunofluorescence
  • Enzyme-linked Immunoabsorbent Assay
  • Dot Blot
  • Dot Blot
  • Dot Blot analysis of GFP at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng and (5) 1.23 ng with GFP monoclonal antibody, clone 9F9.F9 (Texas Red) (Cat # MAB23145).
  • Application Image
  • Immunofluorescence
  • Enzyme-linked Immunoabsorbent Assay
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