Mouse monoclonal antibody raised against full length recombinant GFP. The antibody is conjugated with Horseradish peroxidase (HRP).
Immunogen:
Recombinant fusion protein corresponding to full length amino acid sequence (246 amino acids) derived from the jellyfish Aequorea victoria GFP.
Host:
Mouse
Specificity:
This antibody reactivity is observed against recombinant Green Fluorescent Protein. No reaction is seen against RFP.
Form:
Lyophilized
Conjugation:
HRP
Purification:
This antibody was prepared from tissue culture supernatant by protein A affinity chromatography.
Isotype:
IgG1, kappa
Recommend Usage:
ELISA (1:20000-1:80000) Immunohistochemistry (1:500-1:2000) Western Blot (1:2000-1:5000) The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from 20 mM potassium phosphate, 150 mM NaCl, pH 7.2 (10 mg/mL BSA, 0.01% (w/v) sodium azide).
Storage Instruction:
Store vial at 4°C prior to restoration. After reconstitution with 1.0 mL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage. Aliquot to avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.