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Last updated: 2021/8/8

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GFP monoclonal antibody, clone 9F9.F9 (FITC) 

  • Catalog # : MAB23143
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant GFP. The antibody is conjugated with Fluorescein (FITC).
  • Immunogen:
  • Recombinant fusion protein corresponding to full length amino acid sequence (246 amino acids) derived from the jellyfish Aequorea victoria GFP.
  • Host:
  • Mouse
  • Specificity:
  • This antibody reactivity is observed against recombinant Green Fluorescent Protein. No reaction is seen against RFP.
  • Form:
  • Lyophilized
  • Conjugation:
  • FITC
  • Purification:
  • This antibody was prepared from tissue culture supernatant by protein A affinity chromatography.
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • ELISA (1:20000-1:40000)
    Immunofluorescence (1:500-1:2500)
    Western Blot (1:10000-1:25000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 20 mM potassium phosphate, 150 mM NaCl, pH 7.2 (10 mg/mL BSA, 0.01% (w/v) sodium azide).
  • Storage Instruction:
  • Store vial at 4°C prior to restoration.
    After reconstitution with 1.0 mL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
    Aliquot to avoid repeated freezing and thawing.
    Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western Blot analysis of GFP (50 ng) with GFP monoclonal antibody, clone 9F9.F9 (FITC) (Cat # MAB23143).
  • Immunofluorescence
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
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  • Immunofluorescence
  • Enzyme-linked Immunoabsorbent Assay
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