GFP monoclonal antibody, clone 9F9.F9 (FITC)
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More Files
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Western Blot (Recombinant protein)
Western Blot analysis of GFP (50 ng) with GFP monoclonal antibody, clone 9F9.F9 (FITC) (Cat # MAB23143).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant GFP. The antibody is conjugated with Fluorescein (FITC).
Immunogen
Recombinant fusion protein corresponding to full length amino acid sequence (246 amino acids) derived from the jellyfish Aequorea victoria GFP.
Host
Mouse
Specificity
This antibody reactivity is observed against recombinant Green Fluorescent Protein. No reaction is seen against RFP.
Form
Lyophilized
Conjugation
FITC
Purification
This antibody was prepared from tissue culture supernatant by protein A affinity chromatography.
Isotype
IgG1, kappa
Recommend Usage
ELISA (1:20000-1:40000)
Immunofluorescence (1:500-1:2500)
Western Blot (1:10000-1:25000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM potassium phosphate, 150 mM NaCl, pH 7.2 (10 mg/mL BSA, 0.01% (w/v) sodium azide).
Storage Instruction
Store vial at 4°C prior to restoration.
After reconstitution with 1.0 mL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western Blot analysis of GFP (50 ng) with GFP monoclonal antibody, clone 9F9.F9 (FITC) (Cat # MAB23143).Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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