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Last updated: 2021/8/8

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GFP monoclonal antibody, clone 9F9.F9 (Biotin) 

  • Catalog # : MAB23142
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant GFP. The antibody is conjugated with Biotin.
  • Immunogen:
  • Recombinant fusion protein corresponding to full length amino acid sequence (246 amino acids) derived from the jellyfish Aequorea victoria GFP.
  • Host:
  • Mouse
  • Specificity:
  • This antibody reactivity is observed against recombinant Green Fluorescent Protein. No reaction is seen against RFP.
  • Form:
  • Lyophilized
  • Conjugation:
  • Biotin
  • Purification:
  • This antibody was prepared from tissue culture supernatant by protein A affinity chromatography.
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • ELISA (1:50000-1:200000)
    Immunohistochemistry (1:1000-1:5000)
    Western Blot (1:2000-1:10000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 20 mM potassium phosphate, 150 mM NaCl, pH 7.2 (10 mg/mL BSA, 0.01% (w/v) sodium azide).
  • Storage Instruction:
  • Store vial at 4°C prior to restoration.
    After reconstitution with 1.0 mL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
    Aliquot to avoid repeated freezing and thawing.
    Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Immunohistochemistry
  • Immunohistochemistry
  • Immunohistochemical staining of GFP with GFP monoclonal antibody, clone 9F9.F9 (Biotin) (Cat # MAB23142) at 1:5000 working concentration.
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Western Blot
  • Enzyme-linked Immunoabsorbent Assay
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