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Last updated: 2021/8/8

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GFP monoclonal antibody, clone 9F9.F9 (Alkaline Phosphatase) 

  • Catalog # : MAB23141
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant GFP. The antibody is conjugated with Alkaline Phosphatase (Calf Intestine).
  • Immunogen:
  • Recombinant fusion protein corresponding to full length amino acid sequence (246 amino acids) derived from the jellyfish Aequorea victoria GFP.
  • Host:
  • Mouse
  • Specificity:
  • This antibody reactivity is observed against recombinant Green Fluorescent Protein. No reaction is seen against RFP.
  • Form:
  • Liquid
  • Conjugation:
  • Alkaline Phosphatase
  • Purification:
  • This antibody was prepared from tissue culture supernatant by protein A affinity chromatography.
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • ELISA (1:2000-1:12000)
    Immunohistochemistry (1:200-1:1000)
    Western Blot (1:500-1:2500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 50 mM Tris-Cl, 150 mM NaCl, 1 mM MgCl2, 0.1 mM ZnCl2, pH 8.0 (50% (v/v) glycerol, 10 mg/mL BSA, 0.01% (w/v) sodium azide)
  • Storage Instruction:
  • Store at 4°C before opening. Do not freeze.
    This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western Blot analysis of GFP (100 ng) with GFP monoclonal antibody, clone 9F9.F9 (Alkaline Phosphatase) (Cat # MAB23141).
  • Immunohistochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry
  • Enzyme-linked Immunoabsorbent Assay
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