GFP monoclonal antibody, clone 9F9.F9 (Alkaline Phosphatase)
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More Files
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Western Blot (Recombinant protein)
Western Blot analysis of GFP (100 ng) with GFP monoclonal antibody, clone 9F9.F9 (Alkaline Phosphatase) (Cat # MAB23141).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant GFP. The antibody is conjugated with Alkaline Phosphatase (Calf Intestine).
Immunogen
Recombinant fusion protein corresponding to full length amino acid sequence (246 amino acids) derived from the jellyfish Aequorea victoria GFP.
Host
Mouse
Specificity
This antibody reactivity is observed against recombinant Green Fluorescent Protein. No reaction is seen against RFP.
Form
Liquid
Conjugation
Alkaline Phosphatase
Purification
This antibody was prepared from tissue culture supernatant by protein A affinity chromatography.
Isotype
IgG1, kappa
Recommend Usage
ELISA (1:2000-1:12000)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2500)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Cl, 150 mM NaCl, 1 mM MgCl2, 0.1 mM ZnCl2, pH 8.0 (50% (v/v) glycerol, 10 mg/mL BSA, 0.01% (w/v) sodium azide)
Storage Instruction
Store at 4°C before opening. Do not freeze.
This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western Blot analysis of GFP (100 ng) with GFP monoclonal antibody, clone 9F9.F9 (Alkaline Phosphatase) (Cat # MAB23141).Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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