Hemoglobin monoclonal antibody, clone Hb6
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More Files
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Sandwich ELISA
The sandwich fluoroimmunoassay of Glycated Hemoglobin monoclonal antibody, clone 75C9 (Cat # MAB23139) and Hemoglobin monoclonal antibody, clone Hb6 : Hemoglobin monoclonal antibody, clone Hb6 (Cat # MAB23140) was used as a coating (1 ug/well), Glycated Hemoglobin monoclonal antibody, clone 75C9 (Cat # MAB23139) was labeled with stable Eu3+ chelate and served as detection (0.4 ug/well), native HbA1c was utilized as an antigen. MAb pair Hb6-75C9 was shown to detect specifically native glycated hemoglobin HbA1c.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of hemoglobin.
Immunogen
A synthetic peptide corresponding to the N-terminal end of beta-globin.
Host
Mouse
Reactivity
Human
Specificity
Native human HbA10.
Form
Liquid
Preparation Method
This antibody is produced from a hybridoma have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with glycated (in vitro) synthetic peptide corresponding to the N-terminal end of beta-globin.
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Enzyme Immunoassay
Sandwich ELISA (Hemoglobin monoclonal antibody, clone Hb6 (Cat # MAB23140) works as capture antibody in plate sandwich immunoassay together with Glycated Hemoglobin monoclonal antibody, clone 75C9 (Cat # MAB23139) as detection antibody.)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Sandwich ELISA
The sandwich fluoroimmunoassay of Glycated Hemoglobin monoclonal antibody, clone 75C9 (Cat # MAB23139) and Hemoglobin monoclonal antibody, clone Hb6 : Hemoglobin monoclonal antibody, clone Hb6 (Cat # MAB23140) was used as a coating (1 ug/well), Glycated Hemoglobin monoclonal antibody, clone 75C9 (Cat # MAB23139) was labeled with stable Eu3+ chelate and served as detection (0.4 ug/well), native HbA1c was utilized as an antigen. MAb pair Hb6-75C9 was shown to detect specifically native glycated hemoglobin HbA1c.Enzyme Immunoassay
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