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Last updated: 2022/5/15

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Glycated Hemoglobin monoclonal antibody, clone 75C9 

  • Catalog # : MAB23139
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of glycated hemoglobin.
  • Immunogen:
  • A synthetic peptide corresponding to the N-terminal end of beta-globin.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Specificity:
  • Native human HbA1c, does not cross-react with non-glycated hemoglobin.
  • Form:
  • Liquid
  • Preparation Method:
  • This antibody is produced from a hybridoma have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with glycated (in vitro) synthetic peptide corresponding to the N-terminal end of beta-globin.
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Enzyme-linked Immunoabsorbent Assay
    Enzyme Immunoassay
    Sandwich ELISA (Hemoglobin monoclonal antibody, clone Hb6 (Cat # MAB23140) works as capture antibody in plate sandwich immunoassay together with Glycated Hemoglobin monoclonal antibody, clone 75C9 (Cat # MAB23139) as detection antibody.).
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.09% sodium azide).
  • Storage Instruction:
  • Store at 4°C.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • Specificity of Glycated Hemoglobin monoclonal antibody, clone 75C9 (Cat # MAB23139) interaction with HbA1c checked by direct ELISA: Native HbA1c or HbA10 were used as a coating (100 ng/well), Glycated Hemoglobin monoclonal antibody, clone 75C9 (Cat # MAB23139) was labeled with stable Eu3+ chelate and served as detection, whereas Glycated Hemoglobin monoclonal antibody, clone 75C9 (Cat # MAB23139) detects only HbA1c.
  • Sandwich ELISA
  • Sandwich ELISA
  • The sandwich fluoroimmunoassay of Glycated Hemoglobin monoclonal antibody, clone 75C9 (Cat # MAB23139) and Hemoglobin monoclonal antibody, clone Hb6 (Cat # MAB23140): Hemoglobin monoclonal antibody, clone Hb6 (Cat # MAB23140) was used as a coating (1 ug/well), Glycated Hemoglobin monoclonal antibody, clone 75C9 (Cat # MAB23139) was labeled with stable Eu3+ chelate and served as detection (0.4 ug/well), native HbA1c was utilized as an antigen. MAb pair Hb6-75C9 was shown to detect specifically native glycated hemoglobin HbA1c.
  • Enzyme Immunoassay
  • Application Image
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
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  • Enzyme Immunoassay
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