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Last updated: 2022/5/15

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Anti-Pembrolizumab monoclonal antibody, clone 10E12C6 (Biotin) 

  • Catalog # : MAB23135
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against Pembrolizumab.
    Target gene is PD-1.
  • Immunogen:
  • Pembrolizumab.
  • Host:
  • Mouse
  • Specificity:
  • The product is specific for Pembrolizumab.
  • Form:
  • Lyophilized
  • Conjugation:
  • Biotin
  • Preparation Method:
  • This antibody is produced from a hybridoma resulting from the fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Pembrolizumab.
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • ELISA (ELISA detection: 0.01-0.1 ug/mL) (Direct ELISA)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C on dry atmosphere, lyophilized antibodies are stable at 1 years.
    After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Inhibitory IC50:
  • Non-inhibitory
  • Applications
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • Anti-Pembrolizumab monoclonal antibody, clone 10E12C6 (Biotin) (Cat # MAB23135) binds with Pembrolizumab. Coating antigen: Pembrolizumab, 1 ug/mL. Anti-Pembrolizumab monoclonal antibody, clone 10E12C6 (Biotin) (Cat # MAB23135) dilution start from 1000 ng/mL. EC50 = 5.77 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).
  • Application Image
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5133
  • Gene Name:
  • PDCD1
  • Gene Alias:
  • CD279,PD1,SLEB2,hPD-1,hPD-l
  • Gene Description:
  • programmed cell death 1
  • Gene Summary:
  • This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq
  • Other Designations:
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