Mouse monoclonal antibody raised against Obinutuzumab. Target gene is CD20.
Immunogen:
Obinutuzumab.
Host:
Mouse
Specificity:
The product is specific for Obinutuzumab. The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody Anti-Obinutuzumab monoclonal antibody, clone 18H8 (Cat # MAB23131).
Form:
Lyophilized
Conjugation:
Biotin
Purification:
Protein A purification
Isotype:
IgG2a, kappa
Recommend Usage:
ELISA (ELISA detection: 0.01-1 ug/mL) (Direct/Indirect ELISA) Sandwich ELISA The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from PBS, pH 7.4 (1% BSA, 0.02% sodium azide).
Storage Instruction:
Store at -20°C on dry atmosphere, lyophilized antibodies are stable at 1 years. After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Anti-Obinutuzumab monoclonal antibody, clone 16B7 (Biotin) (Cat # MAB23133) binds with Obinutuzumab. Coating antigen: Obinutuzumab, 1 ug/mL. Anti-Obinutuzumab monoclonal antibody, clone 16B7 (Biotin) (Cat # MAB23133) dilution start from 1000 ng/mL. EC50 = 13.09 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).
Sandwich ELISA
Standard curve of Obinutuzumab Sandwich ELISA. The Obinutuzumab Sandwich ELISA assay is developed by using Anti-Obinutuzumab monoclonal antibody, clone 18H8 (Cat # MAB23131) and Anti-Obinutuzumab monoclonal antibody, clone 16B7 (Biotin) (Cat # MAB23133) as the capture and detection antibodies, respectively. The sensitivity of detecting Obinutuzumab is about 30 pg/mL.
This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq