Anti-Daratumumab monoclonal antibody, clone 139B10
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Specification
Product Description
Rabbit monoclonal antibody raised against Daratumumab.
Target gene is CD38.Immunogen
Daratumumab.
Host
Rabbit
Specificity
The product is specific for Daratumumab.
Form
Lyophilized
Preparation Method
This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Daratumumab.
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Blocking
ELISA (ELISA detection: 0.01-0.1 ug/mL) (Direct/Inhibitory ELISA)
The optimal working dilution should be determined by the end user.Inhibitory IC50
0.017 ug/mL
Storage Buffer
Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
Storage Instruction
Store at -20°C on dry atmosphere, lyophilized antibodies are stable at 1 years.
After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
Anti-Daratumumab monoclonal antibody, clone 139B10 (Cat # MAB23130) binds with Daratumumab. Coating antigen: Daratumumab, 1 ug/mL. Anti-Daratumumab monoclonal antibody, clone 139B10 (Cat # MAB23130) dilution start from 1000 ng/mL. EC50 = 3.092 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).Blocking
Anti-Daratumumab monoclonal antibody, clone 139B10 (Cat # MAB23130) blocks Daratumumab binding with Human CD38 protein. Coating antigen: CD38 protein, 1 ug/mL. Daratumumab-biotin final concentration: 10 ng/mL. Anti-Daratumumab monoclonal antibody, clone 139B10 (Cat # MAB23130) dilution start from 10 ug/mL. IC50 = 17.11 ng/mL. -
Gene Info — CD38
Entrez GeneID
952Gene Name
CD38
Gene Alias
T10
Gene Description
CD38 molecule
Omim ID
107270Gene Ontology
HyperlinkGene Summary
CD38 is a novel multifunctional ectoenzyme widely expressed in cells and tissues especially in leukocytes. CD38 also functions in cell adhesion,signal transduction and calcium signaling. [provided by RefSeq
Other Designations
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase|CD38 antigen|CD38 antigen (p45)|cyclic ADP-ribose hydrolase
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Interactome
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