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Last updated: 2021/4/11

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Anti-Daratumumab monoclonal antibody, clone 139B10 

  • Catalog # : MAB23130
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against Daratumumab.
  • Immunogen:
  • Daratumumab.
  • Host:
  • Rabbit
  • Specificity:
  • The product is specific for Daratumumab.
  • Form:
  • Lyophilized
  • Preparation Method:
  • This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Daratumumab.
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Blocking
    ELISA (ELISA detection: 0.01-0.1 ug/mL) (Direct/Inhibitory ELISA)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C on dry atmosphere, lyophilized antibodies are stable at 1 years.
    After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Inhibitory IC50:
  • 0.017 ug/mL
  • Applications
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • Anti-Daratumumab monoclonal antibody, clone 139B10 (Cat # MAB23130) binds with Daratumumab. Coating antigen: Daratumumab, 1 ug/mL. Anti-Daratumumab monoclonal antibody, clone 139B10 (Cat # MAB23130) dilution start from 1000 ng/mL. EC50 = 3.092 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).
  • Blocking
  • Blocking
  • Anti-Daratumumab monoclonal antibody, clone 139B10 (Cat # MAB23130) blocks Daratumumab binding with Human CD38 protein. Coating antigen: CD38 protein, 1 ug/mL. Daratumumab-biotin final concentration: 10 ng/mL. Anti-Daratumumab monoclonal antibody, clone 139B10 (Cat # MAB23130) dilution start from 10 ug/mL. IC50 = 17.11 ng/mL.
  • Application Image
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
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