Anti-Cetuximab monoclonal antibody, clone 18D5
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Anti-Cetuximab monoclonal antibody, clone 18D5 (Cat # MAB23128) binds with Cetuximab. Coating antigen: Cetuximab, 1 ug/mL. Anti-Cetuximab monoclonal antibody, clone 18D5 (Cat # MAB23128) dilution start from 1250 ng/mL. EC50 = 10.23 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).
Sandwich ELISA
Standard curve of Cetuximab Sandwich ELISA. The Cetuximab Sandwich ELISA assay is developed by using Anti-Cetuximab monoclonal antibody, clone 20H1 (Cat # MAB23129) and Anti-Cetuximab monoclonal antibody, clone 18D5 (Cat # MAB23128) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Cetuximab monoclonal antibody, clone 18D5 (Cat # MAB23128) was labeled with Biotin. The sensitivity of detecting Cetuximab is 0.039 ng/mL.
Blocking
Anti-Cetuximab monoclonal antibody, clone 18D5 (Cat # MAB23128) blocks Cetuximab binding with EGFR. Coating antigen: Cetuximab, 0.5 ug/mL. Anti-Cetuximab monoclonal antibody, clone 18D5 (Cat # MAB23128) final concentration: 15 ng/mL. EGFR dilution start from 10 ug/mL. IC50 = 207.3 ng/mL.
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Specification
Product Description
Mouse monoclonal antibody raised against Cetuximab.
Target gene is EGFR.Immunogen
Cetuximab.
Host
Mouse
Specificity
The product is specific for Cetuximab. This antibody blocks Cetuximab binding with EGFR in ELISA. The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody Anti-Cetuximab monoclonal antibody, clone 20H1 (Cat # MAB23129).
Form
Lyophilized
Purification
Protein A purification
Isotype
IgG1, kappa
Recommend Usage
Blocking
ELISA (ELISA detection: 0.01-1 ug/mL) (Direct/Indirect/Inhibitory ELISA)
Sandwich ELISA
The optimal working dilution should be determined by the end user.Inhibitory IC50
0.2073 ug/mL
Storage Buffer
Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
Storage Instruction
Store at -20°C on dry atmosphere, lyophilized antibodies are stable at 1 years.
After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
Anti-Cetuximab monoclonal antibody, clone 18D5 (Cat # MAB23128) binds with Cetuximab. Coating antigen: Cetuximab, 1 ug/mL. Anti-Cetuximab monoclonal antibody, clone 18D5 (Cat # MAB23128) dilution start from 1250 ng/mL. EC50 = 10.23 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).Sandwich ELISA
Standard curve of Cetuximab Sandwich ELISA. The Cetuximab Sandwich ELISA assay is developed by using Anti-Cetuximab monoclonal antibody, clone 20H1 (Cat # MAB23129) and Anti-Cetuximab monoclonal antibody, clone 18D5 (Cat # MAB23128) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Cetuximab monoclonal antibody, clone 18D5 (Cat # MAB23128) was labeled with Biotin. The sensitivity of detecting Cetuximab is 0.039 ng/mL.Blocking
Anti-Cetuximab monoclonal antibody, clone 18D5 (Cat # MAB23128) blocks Cetuximab binding with EGFR. Coating antigen: Cetuximab, 0.5 ug/mL. Anti-Cetuximab monoclonal antibody, clone 18D5 (Cat # MAB23128) final concentration: 15 ng/mL. EGFR dilution start from 10 ug/mL. IC50 = 207.3 ng/mL. -
Gene Info — EGFR
Entrez GeneID
1956Gene Name
EGFR
Gene Alias
ERBB, ERBB1, HER1, PIG61, mENA
Gene Description
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
Other Designations
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor
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Interactome
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Pathway
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Disease
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