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Last updated: 2021/4/11

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Anti-Cetuximab monoclonal antibody, clone 18D5 

  • Catalog # : MAB23128
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against Cetuximab.
  • Immunogen:
  • Cetuximab.
  • Host:
  • Mouse
  • Specificity:
  • The product is specific for Cetuximab. This antibody blocks Cetuximab binding with EGFR in ELISA. The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody Anti-Cetuximab monoclonal antibody, clone 20H1 (Cat # MAB23129).
  • Form:
  • Lyophilized
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • Blocking
    ELISA (ELISA detection: 0.01-1 ug/mL) (Direct/Indirect/Inhibitory ELISA)
    Sandwich ELISA
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C on dry atmosphere, lyophilized antibodies are stable at 1 years.
    After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Inhibitory IC50:
  • 0.2073 ug/mL
  • Applications
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • Anti-Cetuximab monoclonal antibody, clone 18D5 (Cat # MAB23128) binds with Cetuximab. Coating antigen: Cetuximab, 1 ug/mL. Anti-Cetuximab monoclonal antibody, clone 18D5 (Cat # MAB23128) dilution start from 1250 ng/mL. EC50 = 10.23 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).
  • Sandwich ELISA
  • Sandwich ELISA
  • Standard curve of Cetuximab Sandwich ELISA. The Cetuximab Sandwich ELISA assay is developed by using Anti-Cetuximab monoclonal antibody, clone 20H1 (Cat # MAB23129) and Anti-Cetuximab monoclonal antibody, clone 18D5 (Cat # MAB23128) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Cetuximab monoclonal antibody, clone 18D5 (Cat # MAB23128) was labeled with Biotin. The sensitivity of detecting Cetuximab is 0.039 ng/mL.
  • Blocking
  • Blocking
  • Anti-Cetuximab monoclonal antibody, clone 18D5 (Cat # MAB23128) blocks Cetuximab binding with EGFR. Coating antigen: Cetuximab, 0.5 ug/mL. Anti-Cetuximab monoclonal antibody, clone 18D5 (Cat # MAB23128) final concentration: 15 ng/mL. EGFR dilution start from 10 ug/mL. IC50 = 207.3 ng/mL.
  • Application Image
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
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