Anti-Atezolizumab monoclonal antibody, clone 6B12 (Biotin)
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Enzyme-linked Immunoabsorbent Assay
Anti-Atezolizumab monoclonal antibody, clone 6B12 (Biotin) (Cat # MAB23121) binds with Atezolizumab. Coating antigen: Atezolizumab, 1 ug/mL. Anti-Atezolizumab monoclonal antibody, clone 6B12 (Biotin) dilution start from 1000 ng/mL. EC50 = 8.464 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).
Sandwich ELISA
The Sandwich ELISA assay is developed by using Anti-Atezolizumab monoclonal antibody, clone 10G9 (Cat # MAB23119) and Anti-Atezolizumab monoclonal antibody, clone 6B12 (Biotin) (Cat # MAB23121) as capture and detection antibody, respectively. The sensitivity of detecting Rituximab is about 97 pg/mL.
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Specification
Product Description
Mouse monoclonal antibody raised against Atezolizumab.
Target gene is PD-L1.Immunogen
Atezolizumab.
Host
Mouse
Specificity
The product is specific for Atezolizumab. The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody Anti-Atezolizumab monoclonal antibody, clone 10G9 (Cat # MAB23119).
Form
Lyophilized
Conjugation
Biotin
Preparation Method
This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a mouse immunized with Atezolizumab.
Purification
Protein A purification
Isotype
IgG1, kappa
Recommend Usage
ELISA (0.01-0.1 ug/mL (Direct/Indirect))
Sandwich ELISA
The optimal working dilution should be determined by the end user.Inhibitory IC50
Non-inhibitory
Storage Buffer
Lyophilized from PBS, pH 7.4 (1% BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C on dry atmospher. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
Anti-Atezolizumab monoclonal antibody, clone 6B12 (Biotin) (Cat # MAB23121) binds with Atezolizumab. Coating antigen: Atezolizumab, 1 ug/mL. Anti-Atezolizumab monoclonal antibody, clone 6B12 (Biotin) dilution start from 1000 ng/mL. EC50 = 8.464 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).Sandwich ELISA
The Sandwich ELISA assay is developed by using Anti-Atezolizumab monoclonal antibody, clone 10G9 (Cat # MAB23119) and Anti-Atezolizumab monoclonal antibody, clone 6B12 (Biotin) (Cat # MAB23121) as capture and detection antibody, respectively. The sensitivity of detecting Rituximab is about 97 pg/mL. -
Gene Info — CD274
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Interactome
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Pathway
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Disease
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