Hap1 monoclonal antibody, clone 1B6
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of Hap1 in mouse brain lysate using Hap1 monoclonal antibody, clone 1B6 (Cat # MAB2312).
Immunohistochemistry
Staining of rat hypothalamus using Hap1 monoclonal antibody, clone 1B6 (Cat # MAB2312).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant Hap1.
Immunogen
Recombinant fusion protein corresponding to rat Hap1.
Host
Mouse
Theoretical MW (kDa)
95
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to isoforms A and B. WB analysis where bands can be seen ~95 KDa for isoform A and ~110 KDa for isoform B.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (0.5-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
Immunohistochemistry (Frozen sections) (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 0.05% sodium azide)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of Hap1 in mouse brain lysate using Hap1 monoclonal antibody, clone 1B6 (Cat # MAB2312).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunohistochemistry
Staining of rat hypothalamus using Hap1 monoclonal antibody, clone 1B6 (Cat # MAB2312).Immunoprecipitation
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Gene Info — Hap1
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Publication Reference
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Stigmoid bodies contain type I receptor proteins SorLA/LR11 and sortilin: new perspectives on their function.
Gutekunst CA, Torre ER, Sheng Z, Yi H, Coleman SH, Riedel IB, Bujo H.
The Journal of Histochemistry and Cytochemistry 2003 Jun; 51(6):841.
Application：IEM, IF, IHC, WB, Human, Rat, HeLa cells, Rat brains.
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Targeted disruption of Huntingtin-associated protein-1 (Hap1) results in postnatal death due to depressed feeding behavior.
Chan EY, Nasir J, Gutekunst CA, Coleman S, Maclean A, Maas A, Metzler M, Gertsenstein M, Ross CA, Nagy A, Hayden MR.
Human Molecular Genetics 2002 Apr; 11(8):945.
Application：ICC, IHC, WB-Ti, Mouse, Mouse brains, Mouse hypothalamus.
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Stigmoid bodies contain type I receptor proteins SorLA/LR11 and sortilin: new perspectives on their function.
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