Anti-Sarilumab monoclonal antibody, clone 23H12
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Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Sarilumab monoclonal antibody, clone 23H12 (Cat # MAB23108) with Sarilumab. While the antibody does not recognize the human IgG (data not shown). Coating antigen: Sarilumab, 1 ug/mL. Anti-Sarilumab monoclonal antibody, clone 23H12 (Cat # MAB23108) dilution start from 1000 ng/mL. EC50 = 1.887 ng/mL.
Sandwich ELISA
Standard curve of Sarilumab Sandwich ELISA. The Sarilumab Sandwich ELISA assay is developed by using Anti-Sarilumab monoclonal antibody, clone 26B10 (Cat # MAB23107) and Anti-Sarilumab monoclonal antibody, clone 23H12 (Cat # MAB23108) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Sarilumab monoclonal antibody, clone 23H12 (Cat # MAB23108) was labeled with Biotin. The sensitivity of detecting Sarilumab is up to 0.04 ng/mL.
Blocking
Anti-Sarilumab monoclonal antibody, clone 23H12 (Cat # MAB23108) blocks Sarilumab binding with IL-6R, Human. Coating antigen: IL-6R 1 ug/mL. Sarilumab final concentration: 25 ng/mL. Anti-Sarilumab monoclonal antibody, clone 23H12 (Cat # MAB23108) dilutions start from 1 ug/mL. IC50 = 0.056 ug/mL.
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Specification
Product Description
Mouse monoclonal antibody raised against Sarilumab.
Target gene is IL-6 receptor.Immunogen
Sarilumab.
Host
Mouse
Specificity
The product is specific for Sarilumab. The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody Anti-Sarilumab monoclonal antibody, clone 26B10 (Cat # MAB23107).
Form
Lyophilized
Preparation Method
This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a mouse immunized with Sarilumab.
Purification
Protein A purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA (ELISA detection: 0.01-0.1 ug/mL; ELISA blocking: 0.1-10 ug/mL) (Direct/Indirect/Inhibitory ELISA)
Sandwich ELISA
The optimal working dilution should be determined by the end user.Inhibitory IC50
0.056 ug/mL
Storage Buffer
Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
Storage Instruction
Store at -20°C on dry atmospher. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Sarilumab monoclonal antibody, clone 23H12 (Cat # MAB23108) with Sarilumab. While the antibody does not recognize the human IgG (data not shown). Coating antigen: Sarilumab, 1 ug/mL. Anti-Sarilumab monoclonal antibody, clone 23H12 (Cat # MAB23108) dilution start from 1000 ng/mL. EC50 = 1.887 ng/mL.Sandwich ELISA
Standard curve of Sarilumab Sandwich ELISA. The Sarilumab Sandwich ELISA assay is developed by using Anti-Sarilumab monoclonal antibody, clone 26B10 (Cat # MAB23107) and Anti-Sarilumab monoclonal antibody, clone 23H12 (Cat # MAB23108) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Sarilumab monoclonal antibody, clone 23H12 (Cat # MAB23108) was labeled with Biotin. The sensitivity of detecting Sarilumab is up to 0.04 ng/mL.Blocking
Anti-Sarilumab monoclonal antibody, clone 23H12 (Cat # MAB23108) blocks Sarilumab binding with IL-6R, Human. Coating antigen: IL-6R 1 ug/mL. Sarilumab final concentration: 25 ng/mL. Anti-Sarilumab monoclonal antibody, clone 23H12 (Cat # MAB23108) dilutions start from 1 ug/mL. IC50 = 0.056 ug/mL. -
Gene Info — IL6R
Entrez GeneID
3570Gene Name
IL6R
Gene Alias
CD126, IL-6R-1, IL-6R-alpha, IL6RA, MGC104991
Gene Description
interleukin 6 receptor
Omim ID
147880Gene Ontology
HyperlinkGene Summary
Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. The protein encoded by this gene is a subunit of the receptor complex for IL6. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
CD126 antigen|OTTHUMP00000034320|interleukin 6 receptor alpha subunit
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Interactome
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Pathway
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