Anti-Ranibizumab monoclonal antibody, clone 6C3
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Specification
Product Description
Mouse monoclonal antibody raised against Ranibizumab.
Target gene is VEGFA.Immunogen
Ranibizumab.
Host
Mouse
Specificity
The product is specific for Ranibizumab. The antibody is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay with detection antibody Anti-Ranibizumab monoclonal antibody, clone 10D12 (Cat # MAB23106).
Form
Lyophilized
Preparation Method
This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a mouse immunized with Ranibizumab.
Purification
Protein A purification
Isotype
IgG1, lambda
Recommend Usage
ELISA (ELISA detection: 0.01-0.1 ug/mL) (Direct/Indirect/Inhibitory ELISA)
Sandwich ELISA
The optimal working dilution should be determined by the end user.Inhibitory IC50
0.85 ug/mL
Storage Buffer
Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
Storage Instruction
Store at -20°C on dry atmospher. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Ranibizumab monoclonal antibody, clone 6C3 (Cat # MAB23105) with Ranibizumab. While the antibody does not recognize the human IgG (data not shown). Coating antigen: Ranibizumab, 1 ug/mL. Anti-Ranibizumab monoclonal antibody, clone 6C3 (Cat # MAB23105) dilution start from 1000 ng/mL. EC50 = 6.043 ng/mL.Sandwich ELISA
Standard curve of Ranibizumab Sandwich ELISA. The Ranibizumab Sandwich ELISA assay is developed by using Anti-Ranibizumab monoclonal antibody, clone 6C3 (Cat # MAB23105) and Anti-Ranibizumab monoclonal antibody, clone 10D12 (Cat # MAB23106) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Ranibizumab monoclonal antibody, clone 10D12 (Cat # MAB23106) was labeled with Biotin. The sensitivity of detecting Ranibizumab is up to 0.05 ng/mL.Blocking
Anti-Ranibizumab monoclonal antibody, clone 6C3 (Cat # MAB23105) blocks Ranibizumab binding with VEGF165. Coating antigen: VEGF165 1 ug/mL. Ranibizumab final concentration: 100 ng/mL. Anti-Ranibizumab antibody dilution start from 10 ug/mL. IC50 = 0.848 ug/mL. -
Gene Info — VEGFA
Entrez GeneID
7422Gene Name
VEGFA
Gene Alias
MGC70609, VEGF, VEGF-A, VPF
Gene Description
vascular endothelial growth factor A
Gene Ontology
HyperlinkGene Summary
This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq
Other Designations
vascular endothelial growth factor isoform VEGF165|vascular permeability factor
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Interactome
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Disease
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