Anti-Durvalumab monoclonal antibody, clone 15D6
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More Files
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Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Durvalumab monoclonal antibody, clone 15D6 (Cat # MAB23104) with Durvalumab. While the antibody does not recognize the human IgG (data not shown). Coating antigen: Durvalumab, 1 ug/mL. Anti-Durvalumab monoclonal antibody, clone 15D6 (Cat # MAB23104) dilution start from 1000 ng/mL. EC50 = 7.222 ng/mL.
Blocking
Anti-Durvalumab monoclonal antibody, clone 15D6 (Cat # MAB23104) blocks Durvalumab binding with PD-L1 protein. Coating antigen: Durvalumab, 0.5 ug/mL. PD-L1 final concentration: 30 ng/mL. Anti-Durvalumab monoclonal antibody, clone 15D6 (Cat # MAB23104) dilutions start from 30 ug/mL. IC50 = 1.37 ug/mL.
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Specification
Product Description
Mouse monoclonal antibody raised against Durvalumab.
Target gene is PD-L1.Immunogen
Durvalumab.
Host
Mouse
Specificity
The product is specific for Durvalumab.
Form
Lyophilized
Preparation Method
This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a mouse immunized with Durvalumab.
Purification
Protein A purification
Isotype
IgG2a, kappa
Recommend Usage
ELISA (ELISA detection: 0.01-0.1 ug/mL; ELISA blocking: 5-30 ug/mL) (Direct/Inhibitory ELISA)
The optimal working dilution should be determined by the end user.Inhibitory IC50
1.37 ug/mL
Storage Buffer
Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
Storage Instruction
Store at -20°C on dry atmospher. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Durvalumab monoclonal antibody, clone 15D6 (Cat # MAB23104) with Durvalumab. While the antibody does not recognize the human IgG (data not shown). Coating antigen: Durvalumab, 1 ug/mL. Anti-Durvalumab monoclonal antibody, clone 15D6 (Cat # MAB23104) dilution start from 1000 ng/mL. EC50 = 7.222 ng/mL.Blocking
Anti-Durvalumab monoclonal antibody, clone 15D6 (Cat # MAB23104) blocks Durvalumab binding with PD-L1 protein. Coating antigen: Durvalumab, 0.5 ug/mL. PD-L1 final concentration: 30 ng/mL. Anti-Durvalumab monoclonal antibody, clone 15D6 (Cat # MAB23104) dilutions start from 30 ug/mL. IC50 = 1.37 ug/mL. -
Gene Info — CD274
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Interactome
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Pathway
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Disease
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