Anti-Nivolumab monoclonal antibody, clone 47F6
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More Files
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Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Nivolumab monoclonal antibody, clone 47F6 (Cat # MAB23101) with Nivolumab. While the antibody does not recognize the human IgG Fc fragment (data not shown). Coating antigen: Nivolumab, 1 ug/mL. Anti-Nivolumab monoclonal antibody, clone 47F6 (Cat # MAB23101) dilutions start from 1000 ng/mL. EC50 = 11.82 ng/mL.
Blocking
Anti-Nivolumab monoclonal antibody, clone 47F6 (Cat # MAB23101) blocks Nivolumab binding with Human PD-1 recombinant protein (PD-1 Fc Chimera, Human). Coating antigen: Nivolumab, 1 ug/mL. PD-1-Fc-biotin final concentration: 10 ng/mL. Anti-Nivolumab monoclonal antibody, clone 47F6 (Cat # MAB23101) dilutions start from 10 ug/mL. IC50 = 0.10 ug/mL.
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Specification
Product Description
Rabbit monoclonal antibody raised against Nivolumab.
Target gene is PD-1.Immunogen
Nivolumab.
Host
Rabbit
Specificity
Nivolumab.
Form
Lyophilized
Preparation Method
This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Nivolumab.
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ELISA (ELISA detection: 0.01-0.1 ug/mL) (Direct/Inhibitory ELISA)
The optimal working dilution should be determined by the end user.Inhibitory IC50
0.104 ug/mL
Storage Buffer
Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
Storage Instruction
Store at -20°C on dry atmospher. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Nivolumab monoclonal antibody, clone 47F6 (Cat # MAB23101) with Nivolumab. While the antibody does not recognize the human IgG Fc fragment (data not shown). Coating antigen: Nivolumab, 1 ug/mL. Anti-Nivolumab monoclonal antibody, clone 47F6 (Cat # MAB23101) dilutions start from 1000 ng/mL. EC50 = 11.82 ng/mL.Blocking
Anti-Nivolumab monoclonal antibody, clone 47F6 (Cat # MAB23101) blocks Nivolumab binding with Human PD-1 recombinant protein (PD-1 Fc Chimera, Human). Coating antigen: Nivolumab, 1 ug/mL. PD-1-Fc-biotin final concentration: 10 ng/mL. Anti-Nivolumab monoclonal antibody, clone 47F6 (Cat # MAB23101) dilutions start from 10 ug/mL. IC50 = 0.10 ug/mL. -
Gene Info — PDCD1
Entrez GeneID
5133Gene Name
PDCD1
Gene Alias
CD279, PD1, SLEB2, hPD-1, hPD-l
Gene Description
programmed cell death 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq
Other Designations
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Interactome
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