Mouse monoclonal antibody raised against Tocilizumab.
Immunogen:
Tocilizumab.
Host:
Mouse
Specificity:
The product is specific for Tocilizumab. The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody Anti-Tocilizumab monoclonal antibody, clone 14B10 (Cat # MAB23095).
Form:
Lyophilized
Conjugation:
Biotin
Preparation Method:
This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a mouse immunized with Tocilizumab.
Purification:
Protein A purification
Isotype:
IgG2b
Recommend Usage:
ELISA (ELISA detection: 0.01-0.1 ug/mL) (Direct/Indirect ELISA) Sandwich ELISA The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from PBS, pH 7.4 (1% BSA, 0.02% sodium azide).
Storage Instruction:
Store at -20°C on dry atmospher. The lyophilized product remains stable up to 1 year at -20°C from date of receipt. After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
ELISA binding of Anti-Tocilizumab monoclonal antibody, clone 6C10 (Biotin) (Cat # MAB23097) with Tocilizumab. While the antibody does not recognize the human IgG (data not shown). Coating antigen: Tocilizumab, 1 ug/mL. Anti-Tocilizumab monoclonal antibody, clone 6C10 (Biotin) (Cat # MAB23097) dilutions start from 1000 ng/mL. EC50 = 1.837 ng/mL.
Sandwich ELISA
Standard curve of Tocilizumab Sandwich ELISA. The Tocilizumab Sandwich ELISA assay is developed by using Anti-Tocilizumab monoclonal antibody, clone 6C10 (Cat # MAB23096) and Anti-Tocilizumab monoclonal antibody, clone 6C10 (Biotin) (Cat # MAB23097) as the capture and detection antibodies, respectively. The sensitivity is less than 100 pg/mL.