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Last updated: 2021/4/11

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Anti-Tocilizumab monoclonal antibody, clone 14B10 

  • Catalog # : MAB23095
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against Tocilizumab.
  • Immunogen:
  • Tocilizumab.
  • Host:
  • Mouse
  • Specificity:
  • The product is specific for Tocilizumab. The antibody is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay with detection antibody Anti-Tocilizumab monoclonal antibody, clone 6C10 (Cat # MAB23096).
  • Form:
  • Lyophilized
  • Preparation Method:
  • This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a mouse immunized with Tocilizumab.
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG2b
  • Recommend Usage:
  • ELISA (ELISA detection: 0.01-0.1 ug/mL) (Direct/Indirect ELISA)
    Sandwich ELISA
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C on dry atmospher. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
    After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Inhibitory IC50:
  • Non-inhibitory
  • Applications
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA binding of Anti-Tocilizumab monoclonal antibody, clone 14B10 (Cat # MAB23095) with Tocilizumab. While the antibody does not recognize the human IgG (data not shown).Coating antigen: Tocilizumab, 1 ug/mL. Anti-Tocilizumab monoclonal antibody, clone 14B10 (Cat # MAB23095) dilutions start from 1000 ng/mL. EC50 =1.653 ng/mL.
  • Sandwich ELISA
  • Sandwich ELISA
  • Standard curve of Tocilizumab Sandwich ELISA. The Tocilizumab Sandwich ELISA assay is developed by using Anti-Tocilizumab monoclonal antibody, clone 14B10 (Cat # MAB23095) and Anti-Tocilizumab monoclonal antibody, clone 6C10 (Cat # MAB23096) as the capture and detection antibodies, respectively In this ELISA assay, Anti-Tocilizumab monoclonal antibody, clone 6C10 (Cat # MAB23096) was labeled with Biotin. The sensitivity is less than 100 pg/mL.
  • Application Image
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
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