Anti-Daratumumab monoclonal antibody, clone 6A3
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Daratumumab monoclonal antibody, clone 6A3 (Cat # MAB23093) with Daratumumab. Coating antigen: Daratumumab, 1 ug/mL. Anti-Daratumumab monoclonal antibody, clone 6A3 dilutions start from 1000 ng/mL. EC50 = 22.05 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).
Sandwich ELISA
The Sandwich ELISA assay is developed by using Anti-Daratumumab monoclonal antibody, clone 11F2 (Cat # MAB23092) and Anti-Daratumumab monoclonal antibody, clone 6A3 (Cat # MAB23093) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Daratumumab monoclonal antibody, clone 6A3 was labeled with Biotin. The sensitivity of detecting Daratumumab is up to 0.039 ng/mL.
Blocking
Anti-Daratumumab monoclonal antibody, clone 6A3 (Cat # MAB23093) blocks Daratumumab binding with Human CD38 recombinant protein. Coating antigen: CD38 0.5 ug/mL. Daratumumab final concentration: 60 ng/mL. Anti-Daratumumab antibody dilutions start from 30 ug/mL. IC50 = 0.582 ug/mL.
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Specification
Product Description
Mouse monoclonal antibody raised against Daratumumab.
Target gene is CD38.Immunogen
Daratumumab.
Host
Mouse
Specificity
The product is specific for Daratumumab. The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody : Anti-Daratumumab monoclonal antibody, clone 11F2 (Cat # MAB23092).
Form
Lyophilized
Preparation Method
The antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a mouse immunized with Daratumumab.
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Blocking (10-50 ug/mL)
ELISA (0.01-0.1 ug/mL (Direct/Indirect/Inhibitory))
Sandwich ELISA
The optimal working dilution should be determined by the end user.Inhibitory IC50
0.582 ug/mL
Storage Buffer
Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
Storage Instruction
Store at -20°C. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Daratumumab monoclonal antibody, clone 6A3 (Cat # MAB23093) with Daratumumab. Coating antigen: Daratumumab, 1 ug/mL. Anti-Daratumumab monoclonal antibody, clone 6A3 dilutions start from 1000 ng/mL. EC50 = 22.05 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).Sandwich ELISA
The Sandwich ELISA assay is developed by using Anti-Daratumumab monoclonal antibody, clone 11F2 (Cat # MAB23092) and Anti-Daratumumab monoclonal antibody, clone 6A3 (Cat # MAB23093) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Daratumumab monoclonal antibody, clone 6A3 was labeled with Biotin. The sensitivity of detecting Daratumumab is up to 0.039 ng/mL.Blocking
Anti-Daratumumab monoclonal antibody, clone 6A3 (Cat # MAB23093) blocks Daratumumab binding with Human CD38 recombinant protein. Coating antigen: CD38 0.5 ug/mL. Daratumumab final concentration: 60 ng/mL. Anti-Daratumumab antibody dilutions start from 30 ug/mL. IC50 = 0.582 ug/mL. -
Gene Info — CD38
Entrez GeneID
952Gene Name
CD38
Gene Alias
T10
Gene Description
CD38 molecule
Omim ID
107270Gene Ontology
HyperlinkGene Summary
CD38 is a novel multifunctional ectoenzyme widely expressed in cells and tissues especially in leukocytes. CD38 also functions in cell adhesion,signal transduction and calcium signaling. [provided by RefSeq
Other Designations
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase|CD38 antigen|CD38 antigen (p45)|cyclic ADP-ribose hydrolase
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Interactome
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Pathway
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Disease
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