FBL monoclonal antibody, clone 38F3
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of yeast protein extracts stained with FBL monoclonal antibody, clone 38F3 (Cat # MAB2309). A single band at ~34kDa.
Immunofluorescence
Human SH-SY5Y cells stained with FBL monoclonal antibody, clone 38F3 (Cat # MAB2309), showing prominent specular nucleolar staining. The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue.
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Specification
Product Description
Mouse monoclonal antibody raised against native FBL.
Immunogen
Native purified yeast FBL.
Host
Mouse
Theoretical MW (kDa)
34
Reactivity
Fruit fly, Human, Nematoda, Plants, Rat, Yeast
Specificity
Detects a band of approximately 34 KDa and shows a clear and strong punctate staining of yeast nuclei.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against FBL.
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In tissue culture supernatant (10 mM sodium azide)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of yeast protein extracts stained with FBL monoclonal antibody, clone 38F3 (Cat # MAB2309). A single band at ~34kDa.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Human SH-SY5Y cells stained with FBL monoclonal antibody, clone 38F3 (Cat # MAB2309), showing prominent specular nucleolar staining. The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue. -
Publication Reference
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Imaging native beta-actin mRNA in motile fibroblasts.
Tyagi S, Alsmadi O.
Biophysical Journal 2004 Dec; 87(6):4153.
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Fibrillarin is essential for early development and required for accumulation of an intron-encoded small nucleolar RNA in the mouse.
Newton K, Petfalski E, Tollervey D, Cáceres JF.
Molecular and Cellular Biology 2003 Dec; 23(23):8519.
Application：IF, WB, Mouse, MEFs.
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Identification and characterization of a yeast nucleolar protein that is similar to a rat liver nucleolar protein.
Aris JP, Blobel G.
The Journal of Cell Biology 1988 Jul; 107(1):17.
Application：IEM, IF, RIA, WB-Ce, WB-Ti, Rat, Yeast, Rat liver, Rat liver cells, Yeast cells.
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Imaging native beta-actin mRNA in motile fibroblasts.
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