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Last updated: 2022/7/3

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Anti-Cetuximab monoclonal antibody, clone 69H10 

  • Catalog # : MAB23085
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against Cetuximab.
    Target gene is EGFR.
  • Immunogen:
  • Cetuximab.
  • Host:
  • Rabbit
  • Specificity:
  • The antibody is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay with detection antibody : Anti-Cetuximab monoclonal antibody, clone 90G3 (Cat # MAB23086).
  • Form:
  • Lyophilized
  • Preparation Method:
  • The antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Cetuximab.
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Blocking (10-50 ug/mL)
    ELISA (0.01-0.1 ug/mL (Direct/Indirect/Inhibitory))
    Sandwich ELISA
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
    After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Inhibitory IC50:
  • 0.039 ug/mL
  • Applications
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA binding of Anti-Cetuximab monoclonal antibody, clone 69H10 (Cat # MAB23085) with Cetuximab. Coating antigen: Cetuximab, 1 ug/mL. Anti-Cetuximab monoclonal antibody, clone 69H10 dilutions start from 1000 ng/mL. EC50 = 17.67 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).
  • Sandwich ELISA
  • Sandwich ELISA
  • The Sandwich ELISA assay is developed by using Cetuximab monoclonal antibody, clone 69H10 (Cat # MAB23085) and Anti-Cetuximab monoclonal antibody, clone 90G3 (Cat # MAB23086) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Cetuximab monoclonal antibody, clone 90G3 was labeled with Biotin. The sensitivity of detecting Cetuximab is up to 40 pg/mL.
  • Blocking
  • Blocking
  • Anti-Cetuximab monoclonal antibody, clone 69H10 (Cat # MAB23085) blocks Cetuximab binding with EGFR protein. Coating antigen: EGFR, 0.5 ug/mL. Cetuximab final concentration: 20 ng/mL. The antibody dilutions start from 10 ug/mL. IC50 = 39.3 ng/mL.
  • Application Image
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
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  • Gene Information
  • Entrez GeneID:
  • 1956
  • Gene Name:
  • EGFR
  • Gene Alias:
  • Gene Description:
  • epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • Gene Summary:
  • The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
  • Other Designations:
  • avian erythroblastic leukemia viral (v-erb-b) oncogene homolog,cell growth inhibiting protein 40,cell proliferation-inducing protein 61,epidermal growth factor receptor
  • Interactome
  • Interactome
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