Anti-Atezolizumab monoclonal antibody, clone 43H11
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More Files
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Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Atezolizumab monoclonal antibody, clone 43H11 (Cat # MAB23080) with Atezolizumab. While the antibody does not recognize the human IgG Fc fragment (data not shown). Coating antigen: Atezolizumab, 1 ug/mL. Anti-Atezolizumab monoclonal antibody, clone 43H11 (Cat # MAB23080) dilutions start from 1000 ng/mL. EC50 = 3.749 ng/mL.
Sandwich ELISA
Standard curve of Atezolizumab Sandwich ELISA. The Atezolizumab Sandwich ELISA assay is developed by using Anti-Atezolizumab monoclonal antibody, clone 55D8 (Cat # MAB23079) and Anti-Atezolizumab monoclonal antibody, clone 43H11 (Cat # MAB23080) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Atezolizumab monoclonal antibody, clone 43H11 (Cat # MAB23080) was labeled with Biotin. The sensitivity of detecting Atezolizumab is up to 156 pg/mL.
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Specification
Product Description
Rabbit monoclonal antibody raised against Atezolizumab.
Target gene is PD-L1.Immunogen
Atezolizumab.
Host
Rabbit
Specificity
The antibody is recommended as detection antibody in a pharmacokinetic (PK) bridging assay with captrue antibody Anti-Atezolizumab monoclonal antibody, clone 55D8 (Cat # MAB23079).
Form
Lyophilized
Preparation Method
This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Atezolizumab.
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ELISA (ELISA detection: 0.01-0.1 ug/mL) (Direct/Indirect ELISA)
Sandwich ELISA
The optimal working dilution should be determined by the end user.Inhibitory IC50
0.847 ug/mL
Storage Buffer
Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
Storage Instruction
Store at -20°C. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Atezolizumab monoclonal antibody, clone 43H11 (Cat # MAB23080) with Atezolizumab. While the antibody does not recognize the human IgG Fc fragment (data not shown). Coating antigen: Atezolizumab, 1 ug/mL. Anti-Atezolizumab monoclonal antibody, clone 43H11 (Cat # MAB23080) dilutions start from 1000 ng/mL. EC50 = 3.749 ng/mL.Sandwich ELISA
Standard curve of Atezolizumab Sandwich ELISA. The Atezolizumab Sandwich ELISA assay is developed by using Anti-Atezolizumab monoclonal antibody, clone 55D8 (Cat # MAB23079) and Anti-Atezolizumab monoclonal antibody, clone 43H11 (Cat # MAB23080) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Atezolizumab monoclonal antibody, clone 43H11 (Cat # MAB23080) was labeled with Biotin. The sensitivity of detecting Atezolizumab is up to 156 pg/mL. -
Gene Info — CD274
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Interactome
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