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Last updated: 2022/5/22

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Anti-Bevacizumab monoclonal antibody, clone 46E3 

  • Catalog # : MAB23071
  • Visit Frequency :
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against Bevacizumab.
    Target gene is VEGFA.
  • Immunogen:
  • Bevacizumab.
  • Host:
  • Rabbit
  • Specificity:
  • The product is specific for Bevacizumab. This antibody blocks Bevacizumab binding with VEGF165 (VEGF165, Human). The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody Anti-Bevacizumab monoclonal antibody, clone 30E1 (Cat # MAB23070).
  • Form:
  • Lyophilized
  • Preparation Method:
  • This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Bevacizumab.
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (ELISA detection: 0.01-0.05 ug/mL) (Direct/Indirect/Inhibitory ELISA)
    Sandwich ELISA
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
    After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Inhibitory IC50:
  • 0.046 ug/mL
  • Applications
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA binding of Anti-Bevacizumab monoclonal antibody, clone 46E3 (Cat # MAB23071) with Bevacizumab. While the antibody does not recognize the human IgG Fc fragment (data not shown). In this ELISA assay, Anti-Bevacizumab monoclonal antibody, clone 46E3 (Cat # MAB23071) was labeled with Biotin. Coating antigen: Bevacizumab, 1 ug/mL. Anti-Bevacizumab monoclonal antibody, clone 46E3 (Cat # MAB23071) dilution start from 2000 ng/mL. EC50 = 4.421 ng/mL.
  • Sandwich ELISA
  • Sandwich ELISA
  • Standard curve of Bevacizumab Sandwich ELISA. The Bevacizumab Sandwich ELISA assay is developed by using Anti-Bevacizumab monoclonal antibody, clone 30E1 (Cat # MAB23070) and Anti-Bevacizumab monoclonal antibody, clone 46E3 (Cat # MAB23071) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Bevacizumab monoclonal antibody, clone 46E3 (Cat # MAB23071) was labeled with Biotin. The sensitivity of detecting Bevacizumab is up to 20 pg/mL.
  • Blocking
  • Blocking
  • Anti-Bevacizumab monoclonal antibody, clone 46E3 (Cat # MAB23071) blocking with Vascular Endothelial Growth Factor 165 (VEGF 165). In this ELISA assay, Anti-Bevacizumab monoclonal antibody, clone 46E3 (Cat # MAB23071) was labeled with Biotin. Coating antigen: Bevacizumab, 0.3 ug/mL. Anti-Bevacizumab monoclonal antibody, clone 46E3 (Cat # MAB23071) final concentration: 8 ng/mL. VEGF 165 dilution start from 20 ug/mL.
  • Application Image
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
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  • Gene Information
  • Entrez GeneID:
  • 7422
  • Gene Name:
  • VEGFA
  • Gene Alias:
  • MGC70609,VEGF,VEGF-A,VPF
  • Gene Description:
  • vascular endothelial growth factor A
  • Gene Summary:
  • This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq
  • Other Designations:
  • vascular endothelial growth factor isoform VEGF165,vascular permeability factor
  • Related Disease
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