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EWSR1 monoclonal antibody, clone ARC1674 

  • Catalog # : MAB23064
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human EWSR1.
  • Immunogen:
  • A synthetic peptide corresponding to human EWSR1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 85
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunofluorescence (1:50-1:200)
    Immunohistochemistry (1:50-1:200)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.3 (0.05% BSA, 50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of Lane 1: HeLa, Lane 2: Jurkat, Lane 3: mouse testis, Lane 4: mouse thymus, Lane 5: rat testis using EWSR1 monoclonal antibody, clone ARC1674 (Cat # MAB23064) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of mouse testis using EWSR1 monoclonal antibody, clone ARC1674 (Cat # MAB23064) at dilution of 1:100 (40x lens).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of rat lung using EWSR1 monoclonal antibody, clone ARC1674 (Cat # MAB23064) at dilution of 1:100 (40x lens).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human appendix using EWSR1 monoclonal antibody, clone ARC1674 (Cat # MAB23064) at dilution of 1:100 (40x lens).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of C6 cells using EWSR1 monoclonal antibody, clone ARC1674 (Cat # MAB23064) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of U-2 OS cells using EWSR1 monoclonal antibody, clone ARC1674 (Cat # MAB23064) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of NIH/3T3 cells using EWSR1 monoclonal antibody, clone ARC1674 (Cat # MAB23064) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 2130
  • Gene Name:
  • EWSR1
  • Gene Alias:
  • EWS
  • Gene Description:
  • Ewing sarcoma breakpoint region 1
  • Gene Summary:
  • This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14. [provided by RefSeq
  • Other Designations:
  • Ewings sarcoma EWS-Fli1 (type 1) oncogene,bK984G1.4 (Ewing sarcoma breakpoint region 1 protein)
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