Rabbit monoclonal antibody raised against synthetic peptide of human RPE65.
Immunogen:
A synthetic peptide corresponding to human RPE65.
Host:
Rabbit
Theoretical MW (kDa):
61
Reactivity:
Human, Mouse, Rat
Form:
Liquid
Purification:
Affinity purification
Isotype:
IgG
Recommend Usage:
Immunofluorescence (1:50-1:200) Immunohistochemistry (1:50-1:200) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
Western blot analysis of rat eye using RPE65 monoclonal antibody, clone ARC1659 (Cat # MAB23058) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of rat retina using RPE65 monoclonal antibody, clone ARC1659 (Cat # MAB23058) at dilution of 1:100 (40x lens).
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of mouse retina using RPE65 monoclonal antibody, clone ARC1659 (Cat # MAB23058) at dilution of 1:100 (40x lens).
Immunofluorescence
Immunofluorescent staining of rat eye using RPE65 monoclonal antibody, clone ARC1659 (Cat # MAB23058) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence
Immunofluorescent staining of mouse eye using RPE65 monoclonal antibody, clone ARC1659 (Cat # MAB23058) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
This gene encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated, membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT), the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins, with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa. [provided by RefSeq