Rabbit monoclonal antibody raised against synthetic peptide of human PSMD14.
Immunogen:
A synthetic peptide corresponding to human PSMD14.
Host:
Rabbit
Theoretical MW (kDa):
35
Reactivity:
Human, Mouse, Rat
Form:
Liquid
Purification:
Affinity purification
Isotype:
IgG
Recommend Usage:
Immunofluorescence (1:50-1:200) Immunohistochemistry (1:50-1:200) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
Western blot analysis of Lane 1: HeLa, Lane 2: mouse brain, Lane 3: mouse thymus, Lane 4: rat lung, Lane 5: rat brain using PSMD14 monoclonal antibody, clone ARC1655 (Cat # MAB23055) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of rat ovary using PSMD14 monoclonal antibody, clone ARC1655 (Cat # MAB23055) at dilution of 1:100 (40x lens).
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast using PSMD14 monoclonal antibody, clone ARC1655 (Cat # MAB23055) at dilution of 1:100 (40x lens).
Immunofluorescence
Immunofluorescent staining of C6 cells using PSMD14 monoclonal antibody, clone ARC1655 (Cat # MAB23055) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence
Immunofluorescent staining of U-2 OS cells using PSMD14 monoclonal antibody, clone ARC1655 (Cat # MAB23055) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence
Immunofluorescent staining of NIH/3T3 cells using PSMD14 monoclonal antibody, clone ARC1655 (Cat # MAB23055) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
PSMD14 is a component of the 26S proteasome, a multiprotein complex that degrades proteins targeted for destruction by the ubiquitin pathway (Spataro et al., 1997 [PubMed 9374539]).[supplied by OMIM