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WIPI1 monoclonal antibody, clone ARC1652 

  • Catalog # : MAB23053
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human WIPI1.
  • Immunogen:
  • A synthetic peptide corresponding to human WIPI1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 48
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.3 (0.05% BSA, 50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western Blot analysis of extracts of various cell lines and tissues. Lane 1: BxPC-3, Lane 2: U-87MG, Lane 3: mouse brain, Lane 4: mouse kidney, Lane 5: rat lung, and Lane 6: rat kidney. Primary antibody: at 1:500 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
    Lysates/proteins: 25 ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit.
    Exposure time: 90 s.
  • Application Image
  • Gene Information
  • Gene Name:
  • WIPI1
  • Gene Alias:
  • ATG18,FLJ10055,WIPI49
  • Gene Description:
  • WD repeat domain, phosphoinositide interacting 1
  • Gene Summary:
  • WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI1, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM
  • Other Designations:
  • WD40 repeat protein Interacting with phosphoInositides of 49kDa,WIPI-1 alpha
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