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RNF40 monoclonal antibody, clone ARC1651 

  • Catalog # : MAB23052
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human RNF40.
  • Immunogen:
  • A synthetic peptide corresponding to human RNF40.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 130
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunohistochemistry (1:50-1:200)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.3 (0.05% BSA, 50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western Blot analysis of extracts of mouse brain. Primary antibody: at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
    Lysates/proteins: 25 ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit.
    Exposure time: 30 s.
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of extracts of various cell lines. Lane 1: MCF7, Lane 2: BxPC-3, and Lane 3: A375. Primary antibody: at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
    Lysates/proteins: 25 ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit.
    Exposure time: 3 s.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon carcinoma with RNF40 monoclonal antibody, clone ARC1651 (Cat # MAB23052) at dilution of 1:100 (40x lens).
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 9810
  • Gene Name:
  • RNF40
  • Gene Alias:
  • BRE1B,DKFZp686K191,KIAA0661,MGC13051,RBP95,STARING
  • Gene Description:
  • ring finger protein 40
  • Gene Summary:
  • The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery. [provided by RefSeq
  • Other Designations:
  • 95 kDa retinoblastoma protein binding protein,BRE1 E3 ubiquitin ligase homolog B,Rb-associated protein
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