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Last updated: 2016/11/27
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SIAH1/SIAH2 monoclonal antibody, clone 8G7H12

  • Catalog # : MAB2299
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of SIAH1/SIAH2.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 280-331 of drosophila SIAH1/SIAH2.
  • Host:
  • Mouse
  • Reactivity:
  • Fruit fly, Human, Pig, Rat, Zebra fish
  • Specificity:
  • This antibody is specific to human SIAH 1 and 2 and Drosophila SINA in proliferating cells and stem cells. This antibody does not detect Drosophila SINAH or normal, nondividing cells.
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Western Blot (1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In tissue culture supernatant (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. Do not freeze.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • SIAH1/SIAH2 monoclonal antibody, clone 8G7H12 (Cat # MAB2299) recognize both Drosophila SINA and human SIAH. There are two SINA-like E3 ligases, SINA and SINAH, in Drosophila.
    SIAH1/SIAH2 monoclonal antibody, clone 8G7H12 is highly specific in recognizing both Drosophila SINA and human SIAH proteins but not Drosophila SINAH.
    Coomassie blue staining patterns of both the wild-type (WT) and dominant negative (PD) forms of affinity-purified recombinant GST-SINA/SINAH/SIAH fusion proteins are shown in the top panel.
    Protein standards were included and are shown in kDa.
    Cross-reactivity of SIAH1/SIAH2 monoclonal antibody, clone 8G7H12 against Drosophila SINA and SINAH, and human hSIAH-1 and hSIAH-2 proteins were determined.
    SIAH1/SIAH2 monoclonal antibody, clone 8G7H12 recognizes both human SIAH-1 and SIAH-2 protein.
    Photo courtesy of Dr. Amy Tang, Mayo Clinic Cancer Center.
  • Immunoprecipitation
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunoprecipitation
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