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SUMO4 monoclonal antibody, clone ARC1382 

  • Catalog # : MAB22986
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human SUMO4.
  • Immunogen:
  • A synthetic peptide corresponding to human SUMO4.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 15
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunofluorescence (1:50-1:200)
    Immunohistochemistry (1:50-1:200)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.3 (0.05% BSA, 50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of Lane 1: 293T cell lysate, Lane 2: A-431 cell lysate, Lane 3: Raji cell lysate, Lane 4: mouse lung tissue lysate, Lane 5: mouse brain tissue lysate, Lane 6: rat lung tissue lysate using SUMO4 monoclonal antibody, clone ARC1382 at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of (A) human colon carcinoma, (B) mouse kidney using SUMO4 monoclonal antibody, clone ARC1382 at dilution of 1:100 (40x lens).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of U-2 OS cells using SUMO4 monoclonal antibody, clone ARC1382 at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Gene Name:
  • SUMO4
  • Gene Alias:
  • IDDM5,SMT3H4,SUMO-4,dJ281H8.4
  • Gene Description:
  • SMT3 suppressor of mif two 3 homolog 4 (S. cerevisiae)
  • Gene Summary:
  • This gene is a member of the SUMO gene family. This family of genes encode small ubiquitin-related modifiers that are attached to proteins and control the target proteins' subcellular localization, stability, or activity. The protein described in this record is located in the cytoplasm and specifically modifies IKBA, leading to negative regulation of NF-kappa-B-dependent transcription of the IL12B gene. A specific polymorphism in this SUMO gene, which leads to the M55V substitution, has been associated with type I diabetes. The RefSeq contains this polymorphism. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000017390,SMT3 suppressor of mif two 3 homolog 2,SMT3 suppressor of mif two 3 homolog 4,small ubiquitin-like modifier 4 protein,small ubiquitin-like protein 4
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