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RPTOR monoclonal antibody, clone ARC1375 

  • Catalog # : MAB22983
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human RPTOR.
  • Immunogen:
  • A synthetic peptide corresponding to human RPTOR.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 149
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunofluorescence (1:50-1:200)
    Immunohistochemistry (1:50-1:200)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.3 (0.05% BSA, 50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of Lane 1: 293T cell lysate, Lane 2: RD cell lysate, Lane 3: mouse brain tissue lysate using RPTOR monoclonal antibody, clone ARC1375 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of (A) rat brain, (B) mouse kidney using RPTOR monoclonal antibody, clone ARC1375 at dilution of 1:100 (40x lens).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of NIH-3T3 cells using RPTOR monoclonal antibody, clone ARC1375 at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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  • Gene Information
  • Gene Name:
  • RPTOR
  • Gene Alias:
  • KOG1,Mip1
  • Gene Description:
  • regulatory associated protein of MTOR, complex 1
  • Gene Summary:
  • This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats,regulatory associated protein of mTOR
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