Western blot analysis of Lane 1: Jurkat cell lysate, Lane 2: K-562 cell lysate, Lane 3: mouse lung tissue lysate, Lane 4: mouse spleen tissue lysate, Lane 5: mouse thymus tissue lysate, Lane 6: rat thymus tissue lysate using CXCR5 monoclonal antibody, clone ARC1363 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Immunofluorescence
Immunofluorescence analysis of rat spleen using CXCR5 monoclonal antibody, clone ARC1363 at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
This gene was identified as a gene specifically expressed in Burkitt's lymphoma and lymphatic tissues. The protein encoded by this gene is predicted to be a seven transmembrane G protein- coupled receptor and belongs to the CXC chemokine receptor family. BLC, a B-lymphocyte chemoattractant, was identified to be a specific ligand for this receptor. Studies of this gene and its mouse conterpart strongly suggest the essential function of this gene in B cell migration and localization within specific anatomic compartments, such as follicles in lymph nodes as well as in spleen. Two alternatively spliced variants of this gene exist. [provided by RefSeq