Western blot analysis of Lane 1: C6 cell lysate, Lane 2: U-251 MG cell lysate, Lane 3: mouse testis tissue lysate, Lane 4: mouse brain tissue lysate, Lane 5: rat testis tissue lysate using YWHAQ monoclonal antibody, clone ARC1358 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Immunofluorescence
Immunofluorescence analysis of NIH-3T3 cells using YWHAQ monoclonal antibody, clone ARC1358 at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq
Other Designations:
14-3-3 protein T-cell,14-3-3 protein tau,14-3-3 protein theta,OTTHUMP00000015730,protein tau,tyrosine 3/tryptophan 5 -monooxygenase activation protein, theta polypeptide