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DIABLO monoclonal antibody, clone ARC1340 

  • Catalog # : MAB22969
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human DIABLO.
  • Immunogen:
  • A synthetic peptide corresponding to human DIABLO.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 21
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunofluorescence (1:50-1:200)
    Immunohistochemistry (1:50-1:200)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.3 (0.05% BSA, 50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of Lane 1: SP2/0 cell lysate, Lane 2: HeLa cell lysate, Lane 3: MCF7 cell lysate, Lane 4: C2C12 cell lysate, Lane 5: Jurkat cell lysate, Lane 6: C6 cell lysate, Lane 7: mouse heart tissue lysate, Lane 8: rat lung tissue lysate, Lane 9: rat testis tissue lysate using DIABLO monoclonal antibody, clone ARC1340 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of rat testis using DIABLO monoclonal antibody, clone ARC1340 at dilution of 1:100 (40x lens).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of C6 cells using DIABLO monoclonal antibody, clone ARC1340 at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Gene Name:
  • DIABLO
  • Gene Alias:
  • DIABLO-S,FLJ10537,FLJ25049,SMAC,SMAC3
  • Gene Description:
  • diablo homolog (Drosophila)
  • Gene Summary:
  • This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Four alternatively spliced transcript variants have been described for this gene, with two of them encoding different isoforms and the other two probably not encoding a protein. [provided by RefSeq
  • Other Designations:
  • 0610041G12Rik,diablo,direct IAP-binding protein with low pI,mitochondrial Smac protein,second mitochondria-derived activator of caspase
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