Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: 293T cell lysate, Lane 3: mouse liver tissue lysate, Lane 4: mouse spleen tissue lysate, Lane 5: mouse kidney tissue lysate using TXNRD2 monoclonal antibody, clone ARC1339 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Thioredoxin reductase (TR) is a dimeric NADPH-dependent FAD containing enzyme that catalyzes the reduction of the active site disulfide of thioredoxin and other substrates. TR is a member of a family of pyridine nucleotide-disulfide oxidoreductases and is a key enzyme in the regulation of the intracellular redox environment. Three thioredoxin reductase genes have been found that encode selenocysteine containing proteins. This gene partially overlaps the COMT gene on chromosome 22. [provided by RefSeq