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BUB3 monoclonal antibody, clone ARC1318 

  • Catalog # : MAB22964
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human BUB3.
  • Immunogen:
  • A synthetic peptide corresponding to human BUB3.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 40
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunofluorescence (1:50-1:200)
    Immunohistochemistry (1:50-1:200)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.3 (0.05% BSA, 50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: 293T cell lysate, Lane 3: MCF7 cell lysate, Lane 4: C2C12 cell lysate, Lane 5: mouse liver tissue lysate, Lane 6: rat lung tissue lysate using BUB3 monoclonal antibody, clone ARC1318 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of (A) rat brain, (B) human colon carcinoma and (C) mouse testis using BUB3 monoclonal antibody, clone ARC1318 at dilution of 1:100 (40x lens).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of C6 cells using BUB3 monoclonal antibody, clone ARC1318 at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 9184
  • Gene Name:
  • BUB3
  • Gene Alias:
  • BUB3L,hBUB3
  • Gene Description:
  • budding uninhibited by benzimidazoles 3 homolog (yeast)
  • Gene Summary:
  • This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
  • Other Designations:
  • BUB3 budding uninhibited by benzimidazoles 3 homolog,OTTHUMP00000020679,OTTHUMP00000020680,budding uninhibited by benomyl,budding uninhibited by benzimidazoles 3,mitotic checkpoint component
  • Gene Pathway
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