Western blot analysis of Lane 1: 293T cell lysate, Lane 2: SKOV3 cell lysate, Lane 3: mouse liver tissue lysate using VAMP3 monoclonal antibody, clone ARC1312 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Immunofluorescence
Immunofluorescence analysis of U-2 OS cells using VAMP3 monoclonal antibody, clone ARC1312 at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein encoded by this gene was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation. [provided by RefSeq
Other Designations:
OTTHUMP00000001361,cellubrevin,synaptobrevin-3,vesicle-associated membrane protein 3