Western blot analysis of Lane 1: SH-SY5Y cell lysate, Lane 2: U-251 MG cell lysate, Lane 3: U-87 MG cell lysate, Lane 4: mouse brain tissue lysate, Lane 5: rat brain tissue lysate using NR2E1 monoclonal antibody, clone ARC1295 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.