Slc9a3 (phospho S552) monoclonal antibody, clone 14D5
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of Phospho-Slc9a3 in human kidney lysate using with Slc9a3 (phospho S552) monoclonal antibody, clone 14D5 (Cat # MAB2294).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic phosphopeptide of Slc9a3.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S552 of rat Slc9a3.
Host
Mouse
Reactivity
Human, Mouse, Opossum, Rabbit, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunohistochemistry (1:50-1:1000)
Immunofluorescence (1:50-1:1000)
Western Blot (1-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-glycine, 150 mM NaCl (0.05% sodium azide)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of Phospho-Slc9a3 in human kidney lysate using with Slc9a3 (phospho S552) monoclonal antibody, clone 14D5 (Cat # MAB2294).Immunohistochemistry
Immunofluorescence
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — Slc9a3
Entrez GeneID
24784Gene Name
Slc9a3
Gene Alias
Nhe3
Gene Description
solute carrier family 9 (sodium/hydrogen exchanger), member 3
Gene Ontology
HyperlinkGene Summary
O
Other Designations
Solute carrier family 9 (sodium/hydrogen exchanger 3), antiporter 3, Na+/H+ (amiloride insensitive)|plasma membrane ion transport protein|solute carrier family 9, member 3
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Publication Reference
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Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites.
Kocinsky HS, Girardi AC, Biemesderfer D, Nguyen T, Mentone S, Orlowski J, Aronson PS.
American Journal of Physiology. Renal Physiology 2005 Aug; 289(2):F249.
Application：ELISA, IF, IHC-Fr, WB-Tr, Monkey, Rat, COS-7, NHE3, OKP cells, Rat kidney cortex.
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Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites.
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