Slc9a3 (phospho S605) monoclonal antibody, clone 10A8
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic phosphopeptide of Slc9a3.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S605 of rat Slc9a3.
Host
Mouse
Reactivity
Mouse, Opossum, Rabbit, Rat
Form
Liquid
Isotype
IgG2b
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunofluorescence (1:20)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-glycine, 150 mM NaCl (0.05% sodium azide)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Phospho-Slc9a3 S605 in transfected COS-7 cells (Lane A. untreated, Lane B. forskolin and IBMX treated) with Slc9a3 (phospho S605) monoclonal antibody, clone 10A8 (Cat # MAB2293).Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — Slc9a3
Entrez GeneID
24784Gene Name
Slc9a3
Gene Alias
Nhe3
Gene Description
solute carrier family 9 (sodium/hydrogen exchanger), member 3
Gene Ontology
HyperlinkGene Summary
O
Other Designations
Solute carrier family 9 (sodium/hydrogen exchanger 3), antiporter 3, Na+/H+ (amiloride insensitive)|plasma membrane ion transport protein|solute carrier family 9, member 3
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Publication Reference
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Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites.
Kocinsky HS, Girardi AC, Biemesderfer D, Nguyen T, Mentone S, Orlowski J, Aronson PS.
American Journal of Physiology. Renal Physiology 2005 Aug; 289(2):F249.
Application:ELISA, WB-Tr, Monkey, Rat, COS-7, NHE3, OKP cells.
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Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites.
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