THOP1 monoclonal antibody, clone 4D6
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of THOP1 in HeLa whole cell lysate (30 ug) (Lane 1 : 0.5 ug/mL ; Lane 2 : 2 ug/mL) with THOP1 monoclonal antibody, clone 4D6 (Cat # MAB2290).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of THOP1.
Immunogen
A synthetic peptide corresponding to amino acids 56-72 of human THOP1.
Host
Mouse
Reactivity
Human, Mouse
Specificity
This antibody is specific to thimet oligopeptidase.
Form
Liquid
Isotype
IgG2b
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:400)
Western Blot (1:750-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-glycine, 150 mM NaCl (0.05% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of THOP1 in HeLa whole cell lysate (30 ug) (Lane 1 : 0.5 ug/mL ; Lane 2 : 2 ug/mL) with THOP1 monoclonal antibody, clone 4D6 (Cat # MAB2290).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
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Gene Info — THOP1
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Interactome
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Pathway
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Publication Reference
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Topoisomerase II alpha expression and the benefit of adjuvant chemotherapy for postoperative patients with non-small cell lung cancer.
Yan S, Shun-Chang J, Li C, Jie L, Ya-Li L, Ling-Xiong W.
BMC Cancer 2010 Nov; 10:621.
Application：IHC-P, Human, NSCLC tissue.
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The cytosolic endopeptidase, thimet oligopeptidase, destroys antigenic peptides and limits the extent of MHC class I antigen presentation.
York IA, Mo AX, Lemerise K, Zeng W, Shen Y, Abraham CR, Saric T, Goldberg AL, Rock KL.
Immunity 2003 Mar; 18(3):429.
Application：WB, Human, HeLa cells.
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Major histocompatibility complex class I-presented antigenic peptides are degraded in cytosolic extracts primarily by thimet oligopeptidase.
Saric T, Beninga J, Graef CI, Akopian TN, Rock KL, Goldberg AL.
The Journal of Biological Chemistry 2001 Sep; 276(39):36474.
Application：WB-Ce, WB-Tr, Human, HeLa cells.
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Monoclonal antibodies against the human metalloprotease EC 3.4.24.15 label neurofibrillary tangles in Alzheimer's disease brain.
Conn KJ, Pietropaolo M, Ju ST, Abraham CR.
Journal of Neurochemistry 1996 May; 66(5):2011.
Application：ELISA, IHC-P, WB-Ce, WB-Ti, Human, Monkey, Brains, Hybridoma cultures.
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Topoisomerase II alpha expression and the benefit of adjuvant chemotherapy for postoperative patients with non-small cell lung cancer.
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