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Last updated: 2016/10/16
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WT1 monoclonal antibody, clone 6F-H2

  • Catalog # : MAB2289
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant WT1.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-181 of human WT1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1, kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:400)
    Western Blot (2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris-glycine, 150 mM NaCl (0.05% sodium azide)
  • Storage Instruction:
  • Store at -20°C or -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of WT1 in 293 cell lysate with WT1 monoclonal antibody, clone 6F-H2 (Cat # MAB2289).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry
  • Immunoprecipitation
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 7490
  • Gene Name:
  • WT1
  • Gene Alias:
  • Gene Description:
  • Wilms tumor 1
  • Gene Summary:
  • This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq
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