WT1 monoclonal antibody, clone 6F-H2
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant WT1.
Immunogen
Recombinant protein corresponding to amino acids 1-181 of human WT1.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:400)
Western Blot (2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-glycine, 150 mM NaCl (0.05% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of WT1 in 293 cell lysate with WT1 monoclonal antibody, clone 6F-H2 (Cat # MAB2289).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry
Immunoprecipitation
-
Gene Info — WT1
Entrez GeneID
7490Protein Accession#
P19544Gene Name
WT1
Gene Alias
GUD, WAGR, WIT-2, WT33
Gene Description
Wilms tumor 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq
Other Designations
-
-
Interactome
-
Disease
-
Publication Reference
-
Characterization of monoclonal antibodies directed to the amino-terminus of the WT1, Wilms' tumor suppressor protein.
Rauscher FJ 3rd, Morris JF, Fredericks WJ, Lopez-Guisa J, Balakrishnan C, Jost M, Herlyn M, Rodeck U.
Hybridoma 1998 Apr; 17(2):191.
Application:ELISA, IHC-Fr, IP, WB-Tr, Insect, Mouse, Mouse kidneys, Sf9 cells.
-
Characterization of monoclonal antibodies directed to the amino-terminus of the WT1, Wilms' tumor suppressor protein.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com