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CCNH monoclonal antibody, clone ARC0893 

  • Catalog # : MAB22832
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human CCNH.
  • Immunogen:
  • A synthetic peptide corresponding to human CCNH.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 38
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunofluorescence (1:50-1:200)
    Immunohistochemistry (1:50-1:200)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.3 (50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: HepG2 cell lysate, Lane 3: Jurkat cell lysate, Lane 4: mouse testis tissue lysate, using CCNH monoclonal antibody, clone ARC0893 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of mouse testis using CCNH monoclonal antibody, clone ARC0893 at dilution of 1:100 (40x lens).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of NIH-3T3 cells using CCNH monoclonal antibody, clone ARC0893 at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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  • Gene Information
  • Entrez GeneID:
  • 902
  • Gene Name:
  • CCNH
  • Gene Alias:
  • CAK,p34,p37
  • Gene Description:
  • cyclin H
  • Gene Summary:
  • The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. [provided by RefSeq
  • Other Designations:
  • CDK-activating kinase,MO15-associated protein,cyclin-dependent kinase-activating kinase
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